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JC-1  [Mitochondrial Membrane Potential Probe] [5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazoylcarbocyanine iodide]

Description: JC-1 is a membrane permeable, cationic carbocyanine dye widely used for determining mitochondrial membrane potential in flow cytometry and fluorescent microscopy. This dye can selectively enter the mitochondria where it reversibly changes color as membrane potentials increase. This property is due to the reversible formation of JC-1 aggregates upon membrane polarization that causes shifts in emitted light from 530 nm (Em. JC-1 monomeric form) to 590 nm (Em. of J-aggregate) when excited at 488 nm. Both colors can be detected using filters for FITC and PE/phycoerythrin/rhodamine, respectively. JC-1 is both qualitative, in regards to the shift from green to orange fluorescence emission, and quantitative, as measured by fluorescence intensity, in both filter sets. JC-1 can be used to indicate the initiation of apoptosis. These characteristics make JC-1 a sensitive marker for mitochondrial membrane potential.
Catalog No. 6875
Unit Size 5 mg
Cas No. 3520-43-2
MDL No. MFCD00188475
Mole. Wt. 652.23
Mole. Form C25H27Cl4IN4
Availability In-Stock
Country of Origin USA

C of A

JC-1  [Mitochondrial Membrane Potential Probe] [5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazoylcarbocyanine iodide]
Pricing Information: $121.00 - $132.00/Unit
Units: 1-4
Units: 5-24
Units: 25-49
Units: +50
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Product Information
• A cationic, potential-sensitive dual-emission Fluorescent carbocyanine probe for measuring membrane potential of mitochondrial



Storage Condition

Store 4 to 8oC (Ref)

Protect from Moisture (D)

Protect from Light (L)



Product Specification Information

Catalog number: 6875     

Name:  JC-1

(5,5',6,6'-Tetrachloro-1,1',3,3'-tetraethylbenzimidazolyl   carbocyanine iodide) [CBIC2(3)]

HPLC Purity: ≥90% at 514 nm


Wavelength Maximum λmax: 514 ± 3 nm in Methanol

Extinction Coefficient: 190,000 ± 15,000 cm-1 M-1

Fluorescence Emission Maximum λEm: 531 ± 4 nm in Methanol

TLC:  ≥90%

Appearance/Form: Purple Solid

Solubility: DMSO, DMF


1H-Benzimidazolium, 5,6-dichloro-2-[3-(5,6-dichloro-1,3-diethyl-1,3-dihydro- 2H-benzimidazol-2-ylidene)-1-propenyl]-1,3-diethyl-, iodide, (E)-


Takatani T, Takahashi K, Uozumi Y, Shikata E, Yamamoto Y, Ito T, Matsuda T, Schaffer SW, Fujio Y, Azuma J. Am J Physiol Cell Physiol 287, C949-53 (2004)

Sperelakis N, Daniel EE. Am J Physiol Gastrointest Liver Physiol 286, G234-43 (2004)

L.B. Chen and S.T. Smiley, W.T. Mason, ed. Fluorescent and Luminescent Probes for Biological Activity, 124, (1993)

S.T. Smiley et al. Proc. Natl. Acad. Sci. USA 88, 3671, (1991). 1; L.B. Chen and S.T. Smiley, W.T. Mason, ed. Fluorescent and Luminescent Probes for Biological Activity, 124, (1993)


For Research Use Only. Not for any animal or human therapeutic

or diagnostic use

No Animal source

Regulatory Compliance


JC-1 (5,5',6,6'-Tetrachloro-1,1',3,3'-tetraethylbenzimidazolyl carbocyanine iodide) [CBIC2(3)]

OSHA Hazard

No Known OSHA Hazards

HMIS Classification

Health: 0

Flammability: 0

Physical Hazard: 0

NFPA Rating

Health: 0

Fire:  0

Reactivity: 0

TSCA Status

Not On TSCA Inventory