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DAPI, dilactate [4',6-Diamidino-2-phenylindole, dilactate]

Description: DAPI dilactate is essentially the same as DAPI HCl (#6937) except in their counter ions. The dilactate counter ions make the dye more water soluble. DAPI shows blue fluorescence upon biniding DNA and also can be excited by UV. The blue fluorescent DAPI stain apparrantly associates with the minor groove of dsDNA, preferentially binding to AT clusters. DAPI is an excellent nuclear counterstain, showing a distinct banding pattern in chromosomes. It is also used for cell-cycle studies and mycoplasma detection. DAPI is a very sensitive dye that could be used to detect single nucleic acid molecules. DAPI has been shown to detect a single DNA molecule in solution, and to detect femtograms of DNA in single cells and chloroplasts.
 
Catalog No. 7012
Unit Size 10 mg
Cas No. 28718-91-4
MDL No. MFCD00467366
Mole. Wt. 457.48
Mole. Form C22H27N5O6
Availability In-Stock
Country of Origin USA

C of A

MSDS
DAPI, dilactate  [4',6-Diamidino-2-phenylindole, dilactate]
Pricing Information: $61.00 - $72.00/Unit
Units: 1-4
$72.00/Unit
Units: 5-24
$66.00/Unit
Units: 25-49
$61.00/Unit
Units: +50
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Product Information

DAPI dilactate is more water soluble than DAPl dihydrochloride and is better used for microinjection. DAPI is a very sensitive dye that could be used to detect single nucleic acid molecules. DAPI has been shown to detect a single DNA molecule in solution, and to detect femtograms of DNA in single cells and chloroplasts.

 

Storage Condition

Protect from Light (PL)

Protect from Moisture (PM)

  Store at -20oC (F)

 

 

Analytical Data & Specification

7012

DAPI, dilactate  [4',6-Diamidino-2-phenylindole, dilactate]

 

Standard

Observed

HPLC Purity

≥90%

 

Absorption: λEx

Methanol

348 ± 3 nm

 

λEx

 

 

 

Fluorescence: λEm

Methanol

458 ± 4 nm

 

λEm

 

 

 

Extinction coefficient

28,000 ± 3,000 cm-1M-1

NMR

D2O

 

 

Elemental Analysis

 

 

TLC

≥95%

 

Appearance/Form

Yellow Solid

Solubility

DMSO, DMF, Methanol and Water

 

4′,6-Diamidino-2-phenyindole, dilactate 

Inheritable histone H4 acetylation of somatic chromatins in cloned embryos." Wee G, Koo DB, Song BS, Kim JS, Kang MJ, Moon SJ, Kang YK, Lee KK, Han YM. J Biol Chem 281, 6048-57 (2006)
 
• Spreading of embryologically distinct urothelial cells is inhibited by SPARC." Hudson AE, Feng WC, Delostrinos CF, Carmean N, Bassuk JA. J Cell Physiol 202, 453-63 (2005)
 
Alternative lengthening of telomeres is characterized by high rates of telomeric exchange." Londono-Vallejo JA, Der-Sarkissian H, Cazes L, Bacchetti S, Reddel RR. Cancer Res 64, 2324-7 (2004)

 

 

For Research Use Only. Not for any animal or human therapeutic

or diagnostic use

No Animal source

Regulatory Compliance

7012

DAPI, dilactate  [4',6-Diamidino-2-phenylindole, dilactate]

OSHA Hazard

No Known OSHA Hazards

HMIS Classification

Health: 0

Flammability: 0

Physical Hazard: 0

NFPA Rating

Health: 0

Fire: 0

Reactivity: 0

TSCA Status

Not On TSCA Inventory

Transportation Information

DOT (US)

IMDG

IATA

Not dangerous goods

Not dangerous goods

Not dangerous goods

 


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