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Catalog ID 6009 / 25 mg

CPM

AKA: 7-Diethylamino-3-(4'-maleimidylphenyl)-4-methylcoumarin
Formula Weight CAS#
C24H22N2O4 402.44 76877-33-3
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CPM is a commonly used thiol-reactive blue-fluorescent dye for covalently labeling thiols on biomolecules. CPM is very weakly fluorescent until the maleimide group reacts with a thiol. CPM’s fluorescence enhancement upon reaction allows the quantification of thiols in biological samples by fluorescence without a cleanup step to remove unreacted dye. CPM’s fast reactivity and selectivity for thiols combined with its fluorescence enhancement upon conjugation make this dye a valuable tool for investigations of membrane protein folding and stability. The thiol conjugates of CPM are excited at 384 nm and emit at 470 nm. .

Product Size 25 mg
Form Orange solid
Absorption (estimated) Wavelength (λmax) 384 ± 3 nm (Methanol)
Storage conditions Freeze: Store at -5 to -30°C (F) Protect from moisture (D) Protect from light (L)
MDL# MFCD00077334

* 7-Diethylamino-3-(4'-maleimidylphenyl)-4-methylcoumarin
* 1H-pyrole-2,5-dione,1-[4-[7-(diethylamino)-4-methyl-2-oxo-2H-1-benzopyran-3-yl]phenyl

Fluorescent Probes and Related Products:   Coumarin (Umbelliferone)
Label or Dye:   Coumarin (Umbelliferone) and Methylcoumarin
Reactive Probes and Related Products:   Thiol-Reactive (Haloacetamide/Halobenzyl and Maleimide)

Safety Data Sheet
SDS-PDF
Certificates of Analysis
Lot 52125 04-Oct-2021
Lot 51688 22-Aug-2019
Lot 50943 17-Aug-2016
Lot 50458 19-May-2015
Lot 50053 05-Jun-2014

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