Product 6009
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Catalog ID 6009 / 25 mg
CPM
AKA: 7-Diethylamino-3-(4'-maleimidylphenyl)-4-methylcoumarin
Formula | Weight | CAS# |
---|---|---|
C24H22N2O4 | 402.44 | 76877-33-3 |
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CPM is a commonly used thiol-reactive blue-fluorescent dye for covalently labeling thiols on biomolecules. CPM is very weakly fluorescent until the maleimide group reacts with a thiol. CPM’s fluorescence enhancement upon reaction allows the quantification of thiols in biological samples by fluorescence without a cleanup step to remove unreacted dye. CPM’s fast reactivity and selectivity for thiols combined with its fluorescence enhancement upon conjugation make this dye a valuable tool for investigations of membrane protein folding and stability. The thiol conjugates of CPM are excited at 384 nm and emit at 470 nm. .
Product Size | 25 mg |
---|---|
Form | Orange solid |
Absorption (estimated) | Wavelength (λmax) 384 ± 3 nm (Methanol) |
Storage conditions | Freeze: Store at -5 to -30°C (F) Protect from moisture (D) Protect from light (L) |
MDL# | MFCD00077334 |
* 7-Diethylamino-3-(4'-maleimidylphenyl)-4-methylcoumarin
* 1H-pyrole-2,5-dione,1-[4-[7-(diethylamino)-4-methyl-2-oxo-2H-1-benzopyran-3-yl]phenyl
* 1H-pyrole-2,5-dione,1-[4-[7-(diethylamino)-4-methyl-2-oxo-2H-1-benzopyran-3-yl]phenyl
Fluorescent Probes and Related Products: | Coumarin (Umbelliferone) |
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Label or Dye: | Coumarin (Umbelliferone) and Methylcoumarin |
Reactive Probes and Related Products: | Thiol-Reactive (Haloacetamide/Halobenzyl and Maleimide) |
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