Product 6229

In Stock

Catalog ID 6229 / 10 mg

5-TAMRA single isomer

AKA: 5-Carboxytetramethylrhodamine

Formula	Weight	CAS#
C₂₅H₂₂N₂O₅	430.46	91809-66-4

1-4 - ¤ 115/Unit 5-24 - ¤ 110/Unit

For 25+ Units, Contact us.

5-TAMRA is a purified single isomer, amine-reactive orange-fluorescent dye for synthesis of orange fluorescent biomolecules. This single isomer dye has been widely used in preparing fluorescent peptides, proteins, and enzymes and has extensive applications in immunochemistry. 5-TAMRA carboxylic acid may be reacted with amines on peptides, proteins, modified nucleotides, biopolymers and other amine-containing biomolecules using a carbodiimide like EDAC or DIPC to yield a robust amide linkage. Carboxylic acids may also be used to form ester-linked conjugates or acid-halide products. The absorbance spectrum of some 5-TAMRA conjugates may be dependent on the degree of labeling density and dye-dye interactions, but the fluorescence is insensitive to environment pH and is relatively photostable. Conjugates of 5-TAMRA are excited near 546 nm with fluorescence emission near 580 nm.

Product Size	10 mg
Form	Green Metallic solid
Absorption (estimated)	Wavelength (λmax) 542 ± 3 nm (Methanol)
Fluorescence (estimated)	Wavelength (λmax) 567 ± 4 nm (Methanol)
Storage conditions	Store at Room Temperature (RT) Protect from moisture (D) Protect from light (L)
MDL#	MFCD01073638

* 5-Carboxytetramethylrhodamine
* Xanthylium, 9-(2,4-dicarboxyphenyl)-3,6- bis(dimethylamino)-, inner salt

Amine-Reactive Fluorophores, Biotins and Other Labels:	Carboxylic Acid (Non-Activated, free acid)
Excitation and Emision Ranges:	Excitation: 540-555 nm and Emission: 565-580 nm
Reactive Probes and Related Products:	Non-Activated, Amine-Reactive (Free Acid)
Label or Dye:	Rhodamine / Rosamine / TMR (Tetramethylrhodamine) / TAMRA
Fluorescent Probes and Related Products:	Rhodamine/Rosamine