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Catalog ID 7012 / 10 mg

DAPI, dilactate

AKA: 4',6-Diamidino-2-phenylindole, dilactate
Formula Weight CAS#
C22H27N5O6 457.48 28718-91-4
For 25+ Units, Contact us.

DAPI dilactate is essentially the same as DAPI HCl (#6937) except in their counter ions. The dilactate counter ions make the dye more water soluble. DAPI shows blue fluorescence upon biniding DNA and also can be excited by UV. The blue fluorescent DAPI stain apparrantly associates with the minor groove of dsDNA, preferentially binding to AT clusters. DAPI is an excellent nuclear counterstain, showing a distinct banding pattern in chromosomes. It is also used for cell-cycle studies and mycoplasma detection. DAPI is a very sensitive dye that could be used to detect single nucleic acid molecules. DAPI has been shown to detect a single DNA molecule in solution, and to detect femtograms of DNA in single cells and chloroplasts.

Product Size 10 mg
Form Yellow Solid
Absorption (estimated) Wavelength (λmax) 348 ± 3 nm (Methanol)
Fluorescence (estimated) Wavelength (λmax) 459 ± 4 nm (Methanol)
Storage conditions Freeze: Store at -5 to -30°C (F) Protect from moisture (D) Protect from light (L)
MDL#

* 4',6-Diamidino-2-phenylindole, dilactate
* Indole-6-carboxamidine, 2-(p-amidinophenyl)-, dilactate

Fluorescent/Non-Fluorescent Membrane and Membrane Potential Probes:   Cationic Probes
Cell stains / Cellular Structure Probes:   DNA/RNA Cell Stains
Fluorescent Probes and Related Products:   Other Products

Safety Data Sheet
SDS-PDF
Certificates of Analysis
Lot 50663 04-Nov-2015

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