Product 6007

Monobromobimane is a low molecular weight, non-ionic, fluorescent probe for labeling thiols. This cell-permeant probe is essentially non-fluorescent until it reacts with a thiol group. The bromomethyl group is relatively selective for reaction with thiols at physiological pH but may also react with carboxylates at pH above 7. Monobromobimane is useful to quantify and detect the distribution of glutathione in living cells and tissue homogenates. This low molecular weight label can also be used to derivatize thiol-containing proteins prior to electrophoresis without disturbing the protein’s mobility in the separation. The conjugates of monobromobimane are optimally excited near 394 nm and have a variable emission near 490 nm.