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Catalog ID 6063 / 25 mg

mBCl

AKA: Monochlorobimane
Formula Weight CAS#
C10H11ClN2O2 226.66 76421-73-3
For 25+ Units, Contact us.

Monochlorobimane is a low molecular weight, non-ionic, fluorescent probe for labeling thiols. This cell-permeant probe is essentially non-fluorescent until it reacts with a thiol group. The chloromethyl group is relatively selective for reaction with thiols at physiological pH but may also react with carboxylates at pH above 7. Monochlorobimane is useful to quantify and detect the distribution of glutathione in living cells and tissue homogenates. This low molecular weight label can also be used to derivatize thiol-containing proteins prior to electrophoresis without disturbing the protein’s mobility in the separation. The conjugates of monochlorobimane are optimally excited near 394 nm and have a variable emission near 490 nm.

Product Size 25 mg
Form Solid
Absorption (estimated) Wavelength (λmax) 380 ± 3 nm (Methanol)
Storage conditions Freeze: Store at -5 to -30°C (F) Protect from moisture (D) Protect from light (L)
MDL# MFCD00077379

* Monochlorobimane
* 1H,7H-Pyrazolo(1,2-)pyrazole-1,7-dione, 3-(chloromethyl)-2,5,6-trimethyl-

Fluorescent Probes and Related Products:   Bimane
Reactive Probes and Related Products:   Thiol-Reactive (Haloacetamide/Halobenzyl and Maleimide)

Safety Data Sheet
SDS-PDF
Certificates of Analysis
Lot 51102 16-Feb-2017

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