Product 6875
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Catalog ID 6875 / 5 mg
Other Unit Size: 7983 / 1 Kit: 5 vials Dye and 1 vial CCCP soln
JC-1
AKA: Mitochondrial Membrane Potential Probe
Formula | Weight | CAS# |
---|---|---|
C25H27Cl4IN4 | 652.23 | 3520-43-2 |
For 25+ Units, Contact us.
JC-1 is a membrane permeable, cationic carbocyanine dye widely used for determining mitochondrial membrane potential in flow cytometry and fluorescent microscopy. This dye can selectively enter the mitochondria where it reversibly changes color as membrane potentials increase. This property is due to the reversible formation of JC-1 aggregates upon membrane polarization that causes shifts in emitted light from 530 nm (Em. JC-1 monomeric form) to 590 nm (Em. of J-aggregate) when excited at 488 nm. Both colors can be detected using filters for FITC and PE/phycoerythrin/rhodamine, respectively. JC-1 is both qualitative, in regards to the shift from green to orange fluorescence emission, and quantitative, as measured by fluorescence intensity, in both filter sets. JC-1 can be used to indicate the initiation of apoptosis. These characteristics make JC-1 a sensitive marker for mitochondrial membrane potential.
Product Size | 5 mg |
---|---|
Form | Purple Solid |
Absorption (estimated) | Wavelength (λmax) 514 ± 3 nm (Methanol) |
Fluorescence (estimated) | Wavelength (λmax) 531 ± 4 nm (Methanol) |
Storage conditions | Refrigerate: Store at 4-8°C (Ref) Protect from moisture (D) Protect from light (L) |
MDL# | MFCD00188475 |
* Mitochondrial Membrane Potential Probe
* 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolylcarbocyanine iodide
* 1H-Benzimidazolium, 5,6-dichloro-2-[3-(5,6-dichloro-1,3-diethyl-1,3-dihydro- 2H-benzimidazol-2-ylidene)-1-propenyl]-1,3-diethyl-, iodide,
* 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolylcarbocyanine iodide
* 1H-Benzimidazolium, 5,6-dichloro-2-[3-(5,6-dichloro-1,3-diethyl-1,3-dihydro- 2H-benzimidazol-2-ylidene)-1-propenyl]-1,3-diethyl-, iodide,
Fluorescent Probes and Related Products: | Carbocyanine/Styryl |
---|---|
Fluorescent/Non-Fluorescent Membrane and Membrane Potential Probes: | Membrane Potential Probes |
Cell stains / Cellular Structure Probes: | Mitochondria |
Cell stains / Cellular Structure Probes: | Organelle Stains |
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