Product 6102
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Fura-2, AM is a cell-permeant, fluorescent ion indicator for intracellular calcium ion measurements. Live cells are passively loaded with Fura-2, AM by incubation with the cell membrane-permeant AM esters. Once internalized, cytosolic esterases cleave the AM esters to liberate the active Fura-2 penta-carboxylate ion indicator. Fura-2’s spectral response to ion-binding permits a ratiometric calculation of ion concentration that cancels artifacts of photobleaching, dye concentration gradients, cell thickness and dye leakage. The excitation wavelength of Fura-2 varies with Ca2+ concentration and shifts to shorter wavelengths with increasing calcium concentration. The ratio of fluorescence intensity at 510 nm when excited at 340/380 nm is used for ratiometric calculation of Ca2+ concentration. This is Setareh Biotech's easy-to-use DMSO solution formulated in commonly used 1 mM concentration. .
Product Size | 1 ml |
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Form | Yellow Solid |
Absorption (estimated) | Wavelength (λmax) 370 ± 3 nm (Ethyl Acetate) |
Fluorescence (estimated) | Wavelength (λmax) 475 ± 4 nm (Ethyl Acetate) |
Storage conditions | Freeze: Store at -5 to -30°C (F) Protect from moisture (D) Protect from light (L) |
MDL# | MFCD00036976 |
* Fura-2 acetoxymethyl ester
* 5-Oxazolecarboxylic acid, 2-(6-(bis(2- ((acetyloxy)methoxy)-2-oxoethyl)amino)-5-(2-(2-(bis(2-((acetyloxy)methoxy) -2-oxoethyl)amino)-5-methylphenoxy)ethoxy)-2-benzofuranyl), (acetyloxy)methyl ester
* 5-Oxazolecarboxylic acid, 2-(6-(bis(2- ((acetyloxy)methoxy)-2-oxoethyl)amino)-5-(2-(2-(bis(2-((acetyloxy)methoxy) -2-oxoethyl)amino)-5-methylphenoxy)ethoxy)-2-benzofuranyl), (acetyloxy)methyl ester
Cellular Sensors, Ion and pH Indicators (Fluorescent/Non-Fluorescent): | Fluorescent Calcium Indicators |
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Fluorescent Probes and Related Products: | Fluorescent Indicators (Ca, Cl, K, Mg, Na, Zn) |
Label or Dye: | Fura-2 / Fura-4 / Fura-5 / Fura-FF / Mag-fura |
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