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Catalog ID 6875 / 5 mg

    Other Unit Size:     7983 / 1 Kit: 5 vials Dye and 1 vial CCCP soln   

JC-1

AKA: Mitochondrial Membrane Potential Probe
Formula Weight CAS#
C25H27Cl4IN4 652.23 3520-43-2
For 25+ Units, Contact us.

JC-1 is a membrane permeable, cationic carbocyanine dye widely used for determining mitochondrial membrane potential in flow cytometry and fluorescent microscopy. This dye can selectively enter the mitochondria where it reversibly changes color as membrane potentials increase. This property is due to the reversible formation of JC-1 aggregates upon membrane polarization that causes shifts in emitted light from 530 nm (Em. JC-1 monomeric form) to 590 nm (Em. of J-aggregate) when excited at 488 nm. Both colors can be detected using filters for FITC and PE/phycoerythrin/rhodamine, respectively. JC-1 is both qualitative, in regards to the shift from green to orange fluorescence emission, and quantitative, as measured by fluorescence intensity, in both filter sets. JC-1 can be used to indicate the initiation of apoptosis. These characteristics make JC-1 a sensitive marker for mitochondrial membrane potential.

Product Size 5 mg
Form Purple Solid
Absorption (estimated) Wavelength (λmax) 514 ± 3 nm (Methanol)
Fluorescence (estimated) Wavelength (λmax) 531 ± 4 nm (Methanol)
Storage conditions Refrigerate: Store at 4-8°C (Ref) Protect from moisture (D) Protect from light (L)
MDL# MFCD00188475

* Mitochondrial Membrane Potential Probe
* 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolylcarbocyanine iodide
* 1H-Benzimidazolium, 5,6-dichloro-2-[3-(5,6-dichloro-1,3-diethyl-1,3-dihydro- 2H-benzimidazol-2-ylidene)-1-propenyl]-1,3-diethyl-, iodide,

Fluorescent Probes and Related Products:   Carbocyanine/Styryl
Fluorescent/Non-Fluorescent Membrane and Membrane Potential Probes:   Membrane Potential Probes
Cell stains / Cellular Structure Probes:   Mitochondria
Cell stains / Cellular Structure Probes:   Organelle Stains

Safety Data Sheet
SDS-PDF
Certificates of Analysis
Lot 52452 22-Nov-2023
Lot 50921 01-Aug-2016
Lot 50601 17-Sep-2015
Lot 50191 13-Oct-2014

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