Product 6229
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Catalog ID 6229 / 10 mg
5-TAMRA *single isomer*
AKA: 5-Carboxytetramethylrhodamine
Formula | Weight | CAS# |
---|---|---|
C25H22N2O5 | 430.46 | 91809-66-4 |
For 25+ Units, Contact us.
5-TAMRA is a purified single isomer, amine-reactive orange-fluorescent dye for synthesis of orange fluorescent biomolecules. This single isomer dye has been widely used in preparing fluorescent peptides, proteins, and enzymes and has extensive applications in immunochemistry. 5-TAMRA carboxylic acid may be reacted with amines on peptides, proteins, modified nucleotides, biopolymers and other amine-containing biomolecules using a carbodiimide like EDAC or DIPC to yield a robust amide linkage. Carboxylic acids may also be used to form ester-linked conjugates or acid-halide products. The absorbance spectrum of some 5-TAMRA conjugates may be dependent on the degree of labeling density and dye-dye interactions, but the fluorescence is insensitive to environment pH and is relatively photostable. Conjugates of 5-TAMRA are excited near 546 nm with fluorescence emission near 580 nm.
Product Size | 10 mg |
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Form | Green Metallic solid |
Absorption (estimated) | Wavelength (λmax) 542 ± 3 nm (Methanol) |
Fluorescence (estimated) | Wavelength (λmax) 567 ± 4 nm (Methanol) |
Storage conditions | Store at Room Temperature (RT) Protect from moisture (D) Protect from light (L) |
MDL# | MFCD01073638 |
* 5-Carboxytetramethylrhodamine
* Xanthylium, 9-(2,4-dicarboxyphenyl)-3,6- bis(dimethylamino)-, inner salt
* Xanthylium, 9-(2,4-dicarboxyphenyl)-3,6- bis(dimethylamino)-, inner salt
Amine-Reactive Fluorophores, Biotins and Other Labels: | Carboxylic Acid (Non-Activated, free acid) |
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Excitation and Emision Ranges: | Excitation: 540-555 nm and Emission: 565-580 nm |
Reactive Probes and Related Products: | Non-Activated, Amine-Reactive (Free Acid) |
Label or Dye: | Rhodamine / Rosamine / TMR (Tetramethylrhodamine) / TAMRA |
Fluorescent Probes and Related Products: | Rhodamine/Rosamine |
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