Product 6214
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Fluo-3 AM is a green-fluorescent ion-indicator that is selective for Ca2+ ions. Fluo-3 responds to Ca2+ binding with an increase in fluorescence intensity. Live cells are passively loaded with Fluo-3 AM by incubation with the cell membrane-permeant AM esters. Once internalized, cytosolic esterases cleave the AM esters to liberate the active Fluo-3 tetra-carboxylate ion indicator. After enzymatic hydrolysis, Fluo-3 is optimally excited near 506 nm with fluorescence emission near 526 nm. The longer wavelength of absorption is convenient for minimizing photodamage in cells. Fluo-3 AM is useful for fluorescence microscopy, confocal microscopy, flow cytometry, and HCS applications. Fluo-3, AM has proved particularly valuable for determining GPCR function via cell-based high-throughput screening. Fluo-3 AM has relatively low water solubility and Pluronic F-127 (#6361), a mild detergent, is often used as a dispersing agent to facilitate the loading. .
Product Size | 1 mg |
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Form | Red-orange Solid |
Absorption (estimated) | Wavelength (λmax) 464 ± 3 nm (Methanol) |
Storage conditions | Freeze: Store at -5 to -30°C (F) Protect from moisture (D) Protect from light (L) |
MDL# |
* 4-(6-Acetoxymethoxy-2,7-dichloro-3-oxo-9-xanthenyl)-4'-methyl-2,2'(ethylenedioxy)dianiline-N,N,N',N'-tetraacetic acid tetrakis(acetoxymethyl) ester
Excitation and Emision Ranges: | Excitation: 500-510 nm and Emission: 520-540 nm |
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Label or Dye: | Fluo-3 / Fluo-4 / Fluo-5N |
Fluorescent Probes and Related Products: | Fluorescein, Naphthofluorescein and seminaphthofluorescein |
Cellular Sensors, Ion and pH Indicators (Fluorescent/Non-Fluorescent): | Fluorescent Calcium Indicators |
Fluorescent Probes and Related Products: | Fluorescent Indicators (Ca, Cl, K, Mg, Na, Zn) |
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