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Catalog ID 6078 / 100 mg

Dabcyl, SE

AKA: 4-((4-(Dimethylamino)phenyl)azo)benzoic acid, succinimidyl ester
Formula Weight CAS#
C19H18N4O4 366.38 146998-31-4
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DABCYL SE is an amine-reactive, non-fluorescent, chromophoric dye that is commonly used as a FRET (fluorescence resonance energy transfer) partner with a second dye that is fluorescent. The fluorophore-quencher pairing can be used to synthesize FRET-based nucleic acid probes, FRET-based protease substrates, and other FRET-based enzymatic substrates. These substrates are typically quenched and non-fluorescent until an enzymatic event liberates the fluorophore from the quencher resulting in a fluorescent response. The DABCYL quencher has been paired a variety of commonly used fluorescent dye labels, like FAM (Product 7046), FITC (Product 7023), CR110 (Product 6897), ROX (Product 6254), TAMRA (Product 6197), JOE (Product 7029), EDANS and HEX. DABCYL has a broad visible absorbance spectrum with maximal absorbance at 455 nm and can act as a dark quencher for fluorophores with fluorescence emission between 400 and 550 nm. The activated SE ester is reactive with primary amines on peptides, proteins, modified nucleotides, biopolymers and other amine-containing biomolecules.  .

Product Size 100 mg
Form Red/Orange Solid
Absorption (estimated) Wavelength (λmax) 453 ± 3 nm (Methanol)
Storage conditions Freeze: Store at -5 to -30°C (F) Protect from moisture (D) Protect from light (L)
MDL#

* 4-((4-(Dimethylamino)phenyl)azo)benzoic acid, succinimidyl ester

Reactive Probes and Related Products:   Activated, Amine-Reactive [Isothiocyanate, Succinimydyl Ester (SE), Sulfonyl Halide, TFP Ester, NBD-Cl, NBD-F, etc.]
Amine-Reactive Fluorophores, Biotins and Other Labels:   Active Ester - Succinimidyl Ester (SE or NHS-ester)
Amine-Reactive Fluorophores, Biotins and Other Labels:   Amine-Reactive Quencher
Reactive Probes and Related Products:   Quencher/Non-Fluorescent Probes

Safety Data Sheet
SDS-PDF
Certificates of Analysis
Lot 50795 25-Mar-2016

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