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Catalog ID 6929 / 5 mg


AKA: 5-Carboxyfluorescein diacetate, AM ester
Formula Weight CAS#
C28H20O11 532.45 124412-00-6
For 25+ Units, Contact us.

5-CFDA-AM (5-carboxyfluorescein diacetate, acetoxymethyl ester) is a fluorogenic, cell-permeant esterase dye which is used for cytotoxicity studies, indicating plasma membrane integrity. The dye CFDA-AM is a non-toxic esterase substrate that can be converted by nonspecific esterases of living cells from a membrane permeable, nonpolar, nonfluorescent substance to a polar, (CF). The conversion to CF by the cells indicates the integrity of the plasma membrane, since only an intact membrane can maintain the cytoplasmic milieu which is needed to support esterase activity.

Product Size 5 mg
Form Colorless Solid
Absorption (estimated) Wavelength (λmax) 290 ± 3 nm (Methanol)
Storage conditions Freeze: Store at -5 to -30°C (F) Protect from moisture (D) Protect from light (L)

* 5-Carboxyfluorescein diacetate, AM ester
* Spiro(isobenzofuran-1(3H),9'-(9H) xanthene)-5-carboxylic acid, 3',6'-bis(acetyloxy)-oxo-, (acetyloxy)methyl ester
* 5-Carboxyfluorescein Diacetate, Acetoxymethyl Ester

Enzyme Detection, Substrates and Activity Assays :   Esterase Substrates
Fluorescent Probes and Related Products:   Fluorescein, Naphthofluorescein and seminaphthofluorescein

Safety Data Sheet
Certificates of Analysis
Lot 52458 13-Dec-2023
Lot 52429 26-Sep-2023
Lot 52284 20-Sep-2022
Lot 52167 10-Dec-2021
Lot 52120 22-Sep-2021
Lot 51980 17-Mar-2021
Lot 51929 11-Dec-2020
Lot 51880 09-Oct-2020
Lot 51863 10-Sep-2020
Lot 51819 09-Jun-2020

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