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Catalog ID 6203 / 25 mg

Resorufin β-D-galactopyranoside

Formula Weight CAS#
C18H17NO8 375.33 95079-19-9
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Resorufin β-D-galactopyranoside is a fluorogenic substrate for β-galactosidase. β-galactosidase is a commonly used reporter to monitor for gene expression in molecular biology. Resorufin β-D-galactopyranoside is essentially non-fluorescent until β-galactosidase enzymatic activity releases the fluorescent dye, resorufin. Unlike fluorescein-based substrates, resorufin β-D-galactopyranoside releases the orange-fluorescent resorufin after a single enzymatic hydrolysis event with fast enzyme kinetics, making this substrate useful for flow cytometry, ELISA, and high throughput screening applications. Resorufin β-D-galactopyranoside may be used to detect β-galactosidase enzymatic activity in live cells after permeabilization or may be used to detect β-galactosidase enzymatic activity in cellular extracts. Enzymatic activity is detected by fluorescence with an excitation near 571 nm and fluorescence emission near 585 nm. .

Product Size 25 mg
Form Yellow Orange solid
Absorption (estimated) Wavelength (λmax) 470 ± 3 nm (Buffer pH 10)
Storage conditions Freeze: Store at -5 to -30°C (F) Protect from moisture (D) Protect from light (L)
MDL# MFCD00064055

* 3-phenoxazone 7-(β-D-galactopyranoside)

Enzyme Detection, Substrates and Activity Assays :   Beta-Galactosidase Substrates
Fluorescent Probes and Related Products:   Enzyme Substates
Fluorescent Probes and Related Products:   Resorufin

Safety Data Sheet
SDS-PDF
Certificates of Analysis
Lot 52033 25-May-2021

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