Product 7641
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Catalog ID 7641 / 500 ug
Other Unit Size: 6143 / 10 x 50 ug
Fura-4F, AM *Cell permeable*
Formula | Weight | CAS# |
---|---|---|
C43H44FN3O24 | 1,005.84 | N/A |
For 25+ Units, Contact us.
Fura-4F, AM is a cell-permeant, fluorescent ion indicator for intracellular Ca2+ measurements. Live cells are passively loaded with Fura-4F, AM by incubation with the cell membrane-permeant AM esters. Once internalized, cytosolic esterases cleave the AM esters to liberate the active Fura-4F penta-carboxylate ion indicator. Fura-4F’s spectral response to Ca2+ ion-binding permits a ratiometric calculation of ion concentration that cancels artifacts of photobleaching, dye concentration gradients, cell thickness and dye leakage. The excitation wavelength of Fura-4F varies with Ca2+ concentration and shifts to shorter wavelengths with increasing Ca2+ concentration. The ratio of fluorescence intensity at 510 nm when excited at 340/380 nm is used for ratiometric calculation of Ca2+ ion concentration. Fura-4F is a lower affinity indicator for calcium and is useful for measurements of higher physiological concentrations of calcium ions where a higher affinity indicator would be fully saturated. .
Product Size | 500 ug |
---|---|
Form | Yellow Solid |
Absorption (estimated) | Wavelength (λmax) 370 ± 3 nm (Ethyl Acetate) |
Fluorescence (estimated) | Wavelength (λmax) 475 ± 4 nm (Ethyl Acetate) |
Storage conditions | Freeze: Store at -5 to -30°C (F) Protect from moisture (D) Protect from light (L) |
MDL# |
Cellular Sensors, Ion and pH Indicators (Fluorescent/Non-Fluorescent): | Fluorescent Calcium Indicators |
---|---|
Fluorescent Probes and Related Products: | Fluorescent Indicators (Ca, Cl, K, Mg, Na, Zn) |
Label or Dye: | Fura-2 / Fura-4 / Fura-5 / Fura-FF / Mag-fura |
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