Product 6165

In Stock

Catalog ID 6165 / 1 mg

Other Unit Sizes: 6101 / 20x50 ug 6102 / 1 ml 6103 / 1 mg 8074 / 20 x 50 ug

Fura-2, AM Cell Permeant

AKA: Fura-2 acetoxymethyl ester

Formula	Weight	CAS#
C₄₄H₄₇N₃O₂₄	1,001.87	108964-32-5

1-4 - ¤ 123/Unit 5-24 - ¤ 116/Unit

For 25+ Units, Contact us.

Fura-2, AM is a cell-permeant, fluorescent ion indicator for intracellular calcium ion measurements. Live cells are passively loaded with Fura-2, AM by incubation with the cell membrane-permeant AM esters. Once internalized, cytosolic esterases cleave the AM esters to liberate the active Fura-2 penta-carboxylate ion indicator. Fura-2’s spectral response to ion-binding permits a ratiometric calculation of ion concentration that cancels artifacts of photobleaching, dye concentration gradients, cell thickness and dye leakage. The excitation wavelength of Fura-2 varies with Ca²⁺ concentration and shifts to shorter wavelengths with increasing calcium concentration. The ratio of fluorescence intensity at 510 nm when excited at 340/380 nm is used for ratiometric calculation of Ca²⁺ concentration. .

Product Size	1 mg
Form	Yellow Solid
Absorption (estimated)	Wavelength (λmax) 370 ± 3 nm (Ethyl Acetate)
Fluorescence (estimated)	Wavelength (λmax) 475 ± 4 nm (Ethyl Acetate)
Storage conditions	Freeze: Store at -5 to -30°C (F) Protect from moisture (D) Protect from light (L)
MDL#	MFCD00036976

* Fura-2 acetoxymethyl ester
* 5-Oxazolecarboxylic acid, 2-(6-(bis(2- ((acetyloxy)methoxy)-2-oxoethyl)amino)-5-(2-(2-(bis(2-((acetyloxy)methoxy) -2-oxoethyl)amino)-5-methylphenoxy)ethoxy)-2-benzofuranyl), (acetyloxy)methyl ester

Cellular Sensors, Ion and pH Indicators (Fluorescent/Non-Fluorescent):	Fluorescent Calcium Indicators
Fluorescent Probes and Related Products:	Fluorescent Indicators (Ca, Cl, K, Mg, Na, Zn)
Label or Dye:	Fura-2 / Fura-4 / Fura-5 / Fura-FF / Mag-fura