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Product 6322

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Catalog ID 6322 / 1 g

DiOC2(3)

Formula Weight CAS#
C21H21IN2O2 460.31 905-96-4

DiOC2(3) is a lipophilic, green-fluorescent dye that is commonly used as a membrane potential probe in bacteria and yeasts. Cationic DiOC2(3) accumulates in hyperpolarized cell membranes and aggregates in the lipid bilayer. The formation dye aggregates within the cell membrane leads to a decreased fluorescence intensity and red-shifted fluorescence emission. The red-shifting fluorescence emission has been utilized in ratiometric techniques to estimate cell membrane potentials that are precise and accurate. The fluorescence of these environment-sensitive dyes is greatly enhanced when incorporated into membranes or bound to lipophilic biomolecules such as proteins. They are weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes. DiOC2(3) is excited near 482 nm and has an environmentally dependent fluorescence emission near 497 nm.

Product Size 1 g
Form Red solid
Absorption (estimated) Wavelength (λmax) 482 ± 3 nm (Methanol)
Fluorescence (estimated) Wavelength (λmax) 497 ± 4 nm (Methanol)
Storage conditions Store at Room Temperature (RT) Protect from moisture (D) Protect from light (L)
MDL#

* 3,3'-Diethyloxacarbocyanine iodide
* 3-Ethyl-2-[3-(3-ethyl-2(3H)-benzoxazolylidene)-1-propenyl]benzoxazolium iodide

Fluorescent Probes and Related Products:   Carbocyanine/Styryl
Cell stains / Cellular Structure Probes:   Cell Membranes and Lipids
Fluorescent/Non-Fluorescent Membrane and Membrane Potential Probes:   Membrane Potential Probes

Safety Data Sheet
SDS PDF
Certificates of Analysis
Lot 50430 29-Apr-2015
Lot 50429 22-Jul-2014
Lot 50025 25-Jun-2014
Lot 50029 25-Jun-2014

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