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Product 6214

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Catalog ID 6214 / 1 mg

    Other Unit Sizes:    6136 / 1 mg    6215 / 20 x 50 ug    6217 / 1 ml    6218 / 40 x 1 mg    8065 / 10 x 50 ug   

Fluo-3, AM *Cell Permeant*

Formula Weight CAS#
C51H50Cl2N2O23 1,129.85 121714-22-5

Fluo-3 AM is a green-fluorescent ion-indicator that is selective for Ca2+ ions. Fluo-3 responds to Ca2+ binding with an increase in fluorescence intensity. Live cells are passively loaded with Fluo-3 AM by incubation with the cell membrane-permeant AM esters. Once internalized, cytosolic esterases cleave the AM esters to liberate the active Fluo-3 tetra-carboxylate ion indicator. After enzymatic hydrolysis, Fluo-3 is optimally excited near 506 nm with fluorescence emission near 526 nm. The longer wavelength of absorption is convenient for minimizing photodamage in cells. Fluo-3 AM is useful for fluorescence microscopy, confocal microscopy, flow cytometry, and HCS applications. Fluo-3, AM has proved particularly valuable for determining GPCR function via cell-based high-throughput screening. Fluo-3 AM has relatively low water solubility and Pluronic F-127 (#6361), a mild detergent, is often used as a dispersing agent to facilitate the loading. .

Product Size 1 mg
Form Red-orange Solid
Absorption (estimated) Wavelength (λmax) 464 ± 3 nm (Methanol)
Fluorescence (estimated) Wavelength (λmax) 0 ± N/A nm (N/A)
Storage conditions Freeze: Store at -5 to -30°C (F) Protect from moisture (D) Protect from light (L)
MDL#

* 4-(6-Acetoxymethoxy-2,7-dichloro-3-oxo-9-xanthenyl)-4'-methyl-2,2'(ethylenedioxy)dianiline-N,N,N',N'-tetraacetic acid tetrakis(acetoxymethyl) ester

Excitation and Emision Ranges:   Excitation: 500-510 nm and Emission: 520-540 nm
Label or Dye:   Fluo-3 / Fluo-4 / Fluo-5N
Fluorescent Probes and Related Products:   Fluorescein, Naphthofluorescein and seminaphthofluorescein
Cellular Sensors, Ion and pH Indicators (Fluorescent/Non-Fluorescent):   Fluorescent Calcium Indicators
Fluorescent Probes and Related Products:   Fluorescent Indicators (Ca, Cl, K, Mg, Na, Zn)

Safety Data Sheet
SDS PDF
Certificates of Analysis
Lot 50552 08-Aug-2015
Lot 50442 24-May-2014

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