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Catalog ID 6863 / 25 mg


AKA: Dihydroethidium
Formula Weight CAS#
C21H21N3 315.41 104821-25-2
For 25+ Units, Contact us.

Dihydroethidium is reduced form of the commonly used DNA dye ethidium bromide. It has been used extensively in tissue culture experiments to evaluate reactive oxygen species (ROS) production. Dihydroethidium (DHE), by virtue of its ability to freely permeate cell membranes is used extensively to monitor superoxide production. It had long been postulated that DHE upon reaction with superoxide anions forms a red fluorescent product (ethidium) which intercalates with DNA. However, more recent studies have suggested that the product is actually 2-hydroxyethidium. DHE is perhaps the most specific and least problematic dye; as it detects essentially superoxide radicals, is retained well by cells, and may even tolerate mild fixation. Dihydroethidium itself is blue fluorescent in cell cytoplasm while the oxidized form ethidium is red fluorescent upon DNA intercalation.

Product Size 25 mg
Form pink solid
Absorption (estimated) Wavelength (λmax) 355 ± 3 nm (Acetonitrile)
Storage conditions Freeze: Store at -5 to -30°C (F) Protect from moisture (D) Protect from light (L) Air sensitive (A)
MDL# MFCD00077335

* Dihydroethidium
* Hydroethidine
* 7-Diamino-10-ethyl-9-phenyl-9,10-dihydrophenanthridine

Cell stains / Cellular Structure Probes:   DNA/RNA Cell Stains
Fluorescent Probes and Related Products:   Other Products

Safety Data Sheet
Certificates of Analysis
Lot 52428 25-Sep-2023
Lot 52422 07-Sep-2023
Lot 52290 27-Sep-2022
Lot 50761 01-Feb-2016
Lot 50143 21-Aug-2014
Lot 50239 21-Aug-2014
Lot 50240 21-Aug-2014
Lot 50108 04-Feb-2012

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