Product 6863

In Stock

Catalog ID 6863 / 25 mg

DHE

AKA: Dihydroethidium

Formula	Weight	CAS#
C₂₁H₂₁N₃	315.41	104821-25-2

1-4 - ¤ 129/Unit 5-24 - ¤ 124/Unit

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Dihydroethidium is reduced form of the commonly used DNA dye ethidium bromide. It has been used extensively in tissue culture experiments to evaluate reactive oxygen species (ROS) production. Dihydroethidium (DHE), by virtue of its ability to freely permeate cell membranes is used extensively to monitor superoxide production. It had long been postulated that DHE upon reaction with superoxide anions forms a red fluorescent product (ethidium) which intercalates with DNA. However, more recent studies have suggested that the product is actually 2-hydroxyethidium. DHE is perhaps the most specific and least problematic dye; as it detects essentially superoxide radicals, is retained well by cells, and may even tolerate mild fixation. Dihydroethidium itself is blue fluorescent in cell cytoplasm while the oxidized form ethidium is red fluorescent upon DNA intercalation.

Product Size	25 mg
Form	pink solid
Absorption (estimated)	Wavelength (λmax) 355 ± 3 nm (Acetonitrile)
Storage conditions	Freeze: Store at -5 to -30°C (F) Protect from moisture (D) Protect from light (L) Air sensitive (A)
MDL#	MFCD00077335