Product 6197

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Catalog ID 6197 / 5 mg

6-TAMRA,SE

AKA: 6-Carboxytetramethylrhodamine, succinimidyl ester
Formula Weight CAS#
C29H25N3O7 527.53 150810-69-8

6-TAMRA, SE is a purified single isomer, amine-reactive orange-fluorescent dye for synthesis of orange-fluorescent biomolecules. This single isomer dye has been used extensively in automated DNA and nucleic acid sequencing by capillary electrophoresis. 6-TAMRA, SE has also been used in single-molecule-detection applications as well as capillary electrophoresis of labeled carbohydrates. The activated SE ester is reactive with primary amines on peptides, proteins, modified nucleotides, biopolymers and other amine-containing biomolecules and yields a robust amide linkage. The absorbance spectrum of some 6-TAMRA conjugates may be dependent on the degree of labeling density and dye-dye interactions. Conjugates of 6-TAMRA are excited near 540 nm with fluorescence emission near 564 nm.

Product Size 5 mg
Form Red/Purple Solid
Absorption (estimated) Wavelength (λmax) 547 ± 3 nm (Methanol)
Fluorescence (estimated) Wavelength (λmax) 573 ± 4 nm (Methanol)
Storage conditions Freeze: Store at -5 to -30°C (F) Protect from moisture (D) Protect from light (L)
MDL#

* 6-Carboxytetramethylrhodamine, succinimidyl ester
* Xanthylium, 9-[2-carboxy-5-[[(2,5-dioxo-1- pyrrolidinyl)oxy]carbonyl]phenyl]-3,6- bis(dimethylamino)-, inner salt ]

Reactive Probes and Related Products:   Activated, Amine-Reactive [Isothiocyanate, Succinimydyl Ester (SE), Sulfonyl Halide, TFP Ester, NBD-Cl, NBD-F, etc.]
Amine-Reactive Fluorophores, Biotins and Other Labels:   Active Ester - Succinimidyl Ester (SE or NHS-ester)
Excitation and Emision Ranges:   Excitation: 540-555 nm and Emission: 565-580 nm
Label or Dye:   Rhodamine / Rosamine / TMR (Tetramethylrhodamine) / TAMRA
Fluorescent Probes and Related Products:   Rhodamine/Rosamine

Safety Data Sheet
SDS PDF
Certificates of Analysis
Lot 50202 23-Oct-2014
Lot 50199 21-Oct-2014

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