Product 6134

In Stock

Catalog ID 6134 / 1 mg

Fura-2, pentapotassium salt Cell Impermeant

Formula	Weight	CAS#
C₂₉H₂₂K₅N₃O₁₄	831.99	113694-64-7

1-4 - ¤ 94/Unit 5-24 - ¤ 90/Unit

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Fura-2, pentapotassium salt is a cell-impermeant, fluorescent ion indicator for intracellular calcium ion measurements. Fura-2’s spectral response to ion-binding permits a ratiometric calculation of ion concentration that cancels artifacts of photobleaching, dye concentration gradients, cell thickness and dye leakage. The pentapotassium salt form of Fura-2 is water soluble and may be loaded into live cells by microinjection, patch pipette infusion, scrape loading, bead loading, or ATP-induced permeabilization. The excitation wavelength of Fura-2 varies with calcium ion concentration and shifts to shorter wavelengths with increasing calcium concentration. The ratio of fluorescence intensity at 510 nm when excited at 340/380 nm is used for ratiometric calculation of calcium ion concentration. This is the pentapotassium salt form of Fura-2 for use in applications that may be perturbed by sodium ions. .

Product Size	1 mg
Form	Yellow Solid
Absorption (estimated)	Wavelength (λmax) 354 ± 3 nm (Methanol)
Fluorescence (estimated)	Wavelength (λmax) 457 ± 4 nm (Methanol)
Storage conditions	Freeze: Store at -5 to -30°C (F) Protect from moisture (D) Protect from light (L)
MDL#

* 5-Oxazolecarboxylic acid, 2-(6-(bis(carboxymethyl)amino)-5-(2-(2-(bis(carboxymethyl)amino)-5-methylphenoxy)ethoxy)-2-benzofuranyl)-, pentapotassium salt

Cellular Sensors, Ion and pH Indicators (Fluorescent/Non-Fluorescent):	Fluorescent Calcium Indicators
Fluorescent Probes and Related Products:	Fluorescent Indicators (Ca, Cl, K, Mg, Na, Zn)
Label or Dye:	Fura-2 / Fura-4 / Fura-5 / Fura-FF / Mag-fura