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Catalog ID 6219 / 1mg

Fluo-3, pentapotassium salt *Cell Impermeant*

Formula Weight CAS#
C36H25Cl2K5N2O13 959.99 N/A
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Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fluo-3 and Rhod-2 are most commonly used among the visible light-excitable calcium indicators. Fluo-3 is essentially nonfluorescent unless bound to Ca2+ and exhibits a quantum yield at saturating Ca2+ of ~0.14 and a Kd for Ca2+ of 390 nM.

Product Size 1mg
Form Red Solid
Absorption (estimated) Wavelength (λmax) 506 ± 3 nm (Water)
Storage conditions Freeze: Store at -5 to -30°C (F) Protect from moisture (D) Protect from light (L)
MDL#

Excitation and Emision Ranges:   Excitation: 500-510 nm and Emission: 520-540 nm
Label or Dye:   Fluo-3 / Fluo-4 / Fluo-5N
Fluorescent Probes and Related Products:   Fluorescein, Naphthofluorescein and seminaphthofluorescein
Cellular Sensors, Ion and pH Indicators (Fluorescent/Non-Fluorescent):   Fluorescent Calcium Indicators
Fluorescent Probes and Related Products:   Fluorescent Indicators (Ca, Cl, K, Mg, Na, Zn)

Safety Data Sheet
SDS-PDF
Certificates of Analysis
Lot 51383 20-Apr-2018

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