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Catalog ID 6281 / 25 mg

IANBD Amide

AKA: N,N'-dimethyl-N-(iodoacetyl)-N'-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)ethylenediamine
Formula Weight CAS#
C12H14IN5O4 419.18 173485-12-6
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IANBD Amide is a thiol-reactive green-fluorescent dye that is relatively selective for labeling thiols on proteins, peptides, and other thiol-containing biomolecules. Selective reaction with thiols in the presence of amines may be promoted by performing the labeling reaction at pH less than 7. Conjugates of IANBD Amide are excited near 480 nm and have an environmentally dependent fluorescence emission near 543 nm. The fluorescence emission of IANBD Amide labeled biomolecules is environmentally sensitive and this property been exploited for investigations of conformational changes, ligand binding, and protein structure.

Product Size 25 mg
Form Orange Solid
Absorption (estimated) Wavelength (λmax) 480 ± 3 nm (Methanol)
Fluorescence (estimated) Wavelength (λmax) 543 ± 4 nm (Methanol)
Storage conditions Freeze: Store at -5 to -30°C (F) Protect from moisture (D) Protect from light (L)
MDL#

* N,N'-dimethyl-N-(iodoacetyl)-N'-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)ethylenediamine
* Acetamide, 2-iodo-N-methyl-N-[2-[methyl(7-nitro-2,1,3-benzoxadiazol- 4-yl)amino]ethyl]-

Fluorescent/Non-Fluorescent Membrane and Membrane Potential Probes:   Environment-Sensitive probes
Fluorescent Probes and Related Products:   NBD
Reactive Probes and Related Products:   Thiol-Reactive (Haloacetamide/Halobenzyl and Maleimide)

Safety Data Sheet
SDS-PDF
Certificates of Analysis
Lot 52459 14-Dec-2023
Lot 52399 23-Jun-2023
Lot 52099 17-Aug-2021
Lot 51551 03-Jan-2019
Lot 50948 19-Aug-2016
Lot 50420 20-Apr-2015
Lot 50223 19-Nov-2014

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