Product 6021
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Catalog ID 6021 / 250 mg
3-Cyanoumbelliferone
AKA: 3-Cyano-7-hydroxycoumarin
Formula | Weight | CAS# |
---|---|---|
C10H5NO3 | 187.16 | 19088-73-4 |
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3-Cyanoumbelliferone is a blue-fluorescent dye that is commonly used as a reference standard in Cytochrome P450 enzymatic activity assays. The ethyl ether of 3-Cyanoumbelliferone, 3-Cyano-7-ethoxycoumarin (Product 6099), is a commonly used fluorescent substrate for Cytochrome P450 isoform CYP1A1. 3-Cyanoumbelliferone has a lower pKa than related coumarin dyes and is fluorescent at neutral pH, making this dye a preferred fluorophore for enzymatic assays performed at physiological pH. 3-Cyanoumbelliferone is excited at near 408 nm with emission near 450 nm.
Product Size | 250 mg |
---|---|
Form | Yellow-Orange solid |
Absorption (estimated) | Wavelength (λmax) 408 ± 3 nm (Buffer pH 10) |
Fluorescence (estimated) | Wavelength (λmax) 452 ± 4 nm (*Buffer pH 10) |
Storage conditions | Freeze: Store at -5 to -30°C (F) Protect from moisture (D) Protect from light (L) |
MDL# | MFCD00037480 |
* 3-Cyano-7-hydroxycoumarin
Fluorescent Probes and Related Products: | Coumarin (Umbelliferone) |
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Label or Dye: | Coumarin (Umbelliferone) and Methylcoumarin |
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