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Catalog ID 6739 / 100 mg

    Other Unit Sizes:    7907 / 500 mg    8058 / 100 mg   

D-Biotin, SE

AKA: D-Biotin, succinimidyl ester
Formula Weight CAS#
C14H19N3O5S 341.38 35013-72-0
For 25+ Units, Contact us.

biotinylation is the process of covalently attaching biotin to a protein, nucleic acid or other molecule. Biotinylation is rapid, specific and is unlikely to perturb the natural function of the molecule due to the small size of biotin (MW = 244.31). Biotin binds to streptavidin and avidin with an extremely high affinity, fast on-rate, and high specificity, and these interactions are exploited in many areas of biotechnology to isolate biotinylated molecules of interest. N-hydroxysuccinimide (NHS) esters have poor solubility in aqueous solutions. For reactions in aqueous solution, they must first be dissolved in an organic solvent, then diluted into the aqueous reaction mixture. The most commonly used organic solvents for this purpose are dimethyl sulfoxide (DMSO) and dimethyl formamide (DMF), which are compatible with most proteins at low concentrations. Because of the hydrophobicity of NHS-esters, NHS biotinylation reagents can also diffuse through the cell membrane, meaning that they will biotinylate both internal and external components of a cell.

Product Size 100 mg
Form White Solid
Storage conditions Freeze: Store at -5 to -30°C (F) Protect from moisture (D) Protect from light (L)
MDL# MFCD00078531

* D-Biotin, succinimidyl ester
* Link-EZ NHS-Biotin
* EZ-Link NHS-Biotin *TM of ThermoFisher Scientific / Pierce Biotech* [D-Biotin SE] [2,5-pyrrolidinedione, 1-((5-hexahydro-2-oxo-1H-thieno-(3,4-d)-imidazol-4-yl)-1-oxopentyl)oxy)-(3aS-(3a-,4,6a-))-]

Safety Data Sheet
SDS-PDF
Certificates of Analysis
Lot 50780 17-Mar-2016

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