Product 6269

DiI, DiO, DiD and DiR dyes are a family of lipophilic fluorescent stains for labeling membranes. The fluorescence of these environment-sensitive dyes is greatly enhanced when incorporated into membranes or bound to lipophilic biomolecules such as proteins. They are weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes. DiOC6(3) is a lipophilic, cell-permeant green-fluorescent probe that is selective for staining of the mitochondria of live cells (at low concentrations). At higher concentrations, the dye may be used to stain other internal membranes, such as the endoplasmic reticulum. DiOC6(3) has also been used as a photosensitizer in investigations of photodynamic therapies. DiOC6(3) is excited near 484 nm with an environmentally dependent fluorescence emission near 500 nm.