Product 6203
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Catalog ID 6203 / 25 mg
Resorufin β-D-galactopyranoside
Formula | Weight | CAS# |
---|---|---|
C18H17NO8 | 375.33 | 95079-19-9 |
For 25+ Units, Contact us.
Resorufin β-D-galactopyranoside is a fluorogenic substrate for β-galactosidase. β-galactosidase is a commonly used reporter to monitor for gene expression in molecular biology. Resorufin β-D-galactopyranoside is essentially non-fluorescent until β-galactosidase enzymatic activity releases the fluorescent dye, resorufin. Unlike fluorescein-based substrates, resorufin β-D-galactopyranoside releases the orange-fluorescent resorufin after a single enzymatic hydrolysis event with fast enzyme kinetics, making this substrate useful for flow cytometry, ELISA, and high throughput screening applications. Resorufin β-D-galactopyranoside may be used to detect β-galactosidase enzymatic activity in live cells after permeabilization or may be used to detect β-galactosidase enzymatic activity in cellular extracts. Enzymatic activity is detected by fluorescence with an excitation near 571 nm and fluorescence emission near 585 nm. .
Product Size | 25 mg |
---|---|
Form | Yellow Orange solid |
Absorption (estimated) | Wavelength (λmax) 470 ± 3 nm (Buffer pH 10) |
Storage conditions | Freeze: Store at -5 to -30°C (F) Protect from moisture (D) Protect from light (L) |
MDL# | MFCD00064055 |
* 3-phenoxazone 7-(β-D-galactopyranoside)
Enzyme Detection, Substrates and Activity Assays : | Beta-Galactosidase Substrates |
---|---|
Fluorescent Probes and Related Products: | Enzyme Substates |
Fluorescent Probes and Related Products: | Resorufin |
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