Kit 7803
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NucGel Blue Live ReadyUse Reagent is a formulated and ready-to-use solution of the blue-fluorescent DNA stain, Hoechst 33342, trihydrochloride. Hoechst 33342 is a cell-permeant blue-fluorescent dye that exhibits a significant fluorescence enhancement upon binding to A-T regions in the minor grove of double stranded DNA. Hoechst dyes exhibit relatively large Stokes shifts (Excitation/Emission maxima near 350 nm/460 nm) and relatively low cytotoxicity making them suitable for multicolor labeling experiments in live cells. Hoechst 33342 has been used in a variety of applications including nuclear counterstaining in immunofluorescent experiments, investigations of apoptosis pathways, and cell cycle studies. This kit contains 6 x 2.5 mL dropper vials. Just add 2 drops of NucGel Blue Live ReadyUse Reagent per mL of media or physiological buffer for fluorescent nuclear staining in live cells.
Product Size | 6 x 2.5 mL dropper bottles |
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Form | Green/Yellow solid |
Absorption (estimated) | Wavelength (λmax) 358 ± 3 nm (0.2M HCl/MeOH) |
Fluorescence (estimated) | Wavelength (λmax) 517 ± 4 nm (*0.2M HCl/MeOH) |
Storage conditions | Store at Room Temperature (RT) Protect from moisture (D) Protect from light (L) |
MDL# | MFCD00012678 |
* Hoechst 33342 Blue Live ReadyUse Reagent
* NucBlue® Live ReadyProbes® Reagent *TM of MP*
* NucBlue® Live ReadyProbes® Reagent *TM of MP*
Cell stains / Cellular Structure Probes: | DNA/RNA Cell Stains |
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Label or Dye: | Hoechst |
Product Line: | ReadyUse (Known as ReadyProbes, TM of Molecular Probes) |
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