Product 6169
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Catalog ID 6169 / 1 mg
Fura-2, pentasodium salt *Cell Impermeant*
Formula | Weight | CAS# |
---|---|---|
C29H22Na5N3O14 | 751.45 | 96314-98-6 |
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Fura-2, pentasodium salt is a cell-impermeant, fluorescent ion indicator for intracellular calcium ion measurements. Fura-2’s spectral response to ion-binding permits a ratiometric calculation of ion concentration that cancels artifacts of photobleaching, dye concentration gradients, cell thickness and dye leakage. The pentasodium salt form of Fura-2 is water soluble and may be loaded into live cells by microinjection, patch pipette infusion, scrape loading, bead loading, or ATP-induced permeabilization. The excitation wavelength of Fura-2 varies with Ca2+ concentration and shifts to shorter wavelengths with increasing Ca2+ concentration. The ratio of fluorescence intensity at 510 nm when excited at 340/380 nm is used for ratiometric calculation of Ca2+ concentration. This is the pentasodium salt form of Fura-2 for use in applications that may be perturbed by potassium ions. .
Product Size | 1 mg |
---|---|
Form | Off White Solid |
Absorption (estimated) | Wavelength (λmax) 356 ± 3 nm (Methanol) |
Fluorescence (estimated) | Wavelength (λmax) 465 ± 4 nm (Methanol) |
Storage conditions | Freeze: Store at -5 to -30°C (F) Protect from moisture (D) Protect from light (L) |
MDL# |
* 5-Oxazolecarboxylic acid, 2-(6-(bis(carboxymethyl)amino)-5-(2-(2-(bis(carboxymethyl)amino)-5-methylphenoxy)ethoxy)-2-benzofuranyl)-, pentasodium salt
Cellular Sensors, Ion and pH Indicators (Fluorescent/Non-Fluorescent): | Fluorescent Calcium Indicators |
---|---|
Fluorescent Probes and Related Products: | Fluorescent Indicators (Ca, Cl, K, Mg, Na, Zn) |
Label or Dye: | Fura-2 / Fura-4 / Fura-5 / Fura-FF / Mag-fura |
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