Product 6021

In Stock

Catalog ID 6021 / 250 mg

3-Cyanoumbelliferone

AKA: 3-Cyano-7-hydroxycoumarin

Formula	Weight	CAS#
C₁₀H₅NO₃	187.16	19088-73-4

1-4 - ¤ 115/Unit 5-24 - ¤ 110/Unit

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3-Cyanoumbelliferone is a blue-fluorescent dye that is commonly used as a reference standard in Cytochrome P450 enzymatic activity assays. The ethyl ether of 3-Cyanoumbelliferone, 3-Cyano-7-ethoxycoumarin (Product 6099), is a commonly used fluorescent substrate for Cytochrome P450 isoform CYP1A1. 3-Cyanoumbelliferone has a lower pKa than related coumarin dyes and is fluorescent at neutral pH, making this dye a preferred fluorophore for enzymatic assays performed at physiological pH. 3-Cyanoumbelliferone is excited at near 408 nm with emission near 450 nm.

Product Size	250 mg
Form	Yellow-Orange solid
Absorption (estimated)	Wavelength (λmax) 408 ± 3 nm (Buffer pH 10)
Fluorescence (estimated)	Wavelength (λmax) 452 ± 4 nm (*Buffer pH 10)
Storage conditions	Freeze: Store at -5 to -30°C (F) Protect from moisture (D) Protect from light (L)
MDL#	MFCD00037480