Product 6111

In Stock

Catalog ID 6111 / 100 mg

DABMI

AKA: 4-Dimethylaminophenylazophenyl-4'-maleimide

Formula	Weight	CAS#
C₁₈H₁₆N₄O₂	320.35	87963-80-2

1-4 - ¤ 115/Unit 5-24 - ¤ 110/Unit

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DABMI is a thiol-reactive, non-fluorescent, chromophoric dye that is commonly used as a FRET (fluorescence resonance energy transfer) partner with a second dye that is fluorescent. The fluorophore-quencher pairing can be used to synthesize FRET-based nucleic acid probes, FRET-based protease substrates, and other FRET-based enzymatic substrates. These substrates are typically quenched and non-fluorescent until an enzymatic event liberates the fluorophore from the quencher resulting in a fluorescent response. The DABMI quencher has been paired a variety of commonly used fluorescent dye labels, like FAM (Product 7046), FITC (Product 7023), CR110 (Product 6897) ROX (Product 6254), TAMRA (Product 6197), JOE (Product 7029), EDANS and HEX. DABMI has a broad visible absorbance spectrum with maximal absorbance at 418 nm and can act as a dark quencher for fluorophores with fluorescence emission between 400 and 550 nm. The maleimide is relatively selective for reaction with thiols on peptides, proteins, modified nucleotides, biopolymers and other thiol-containing biomolecules when reacted at pH below 7.5. This reagent has also been used as thiol-derivatization agent for analysis of thiol containing peptides. .

Product Size	100 mg
Form	Red-orange Solid
Absorption (estimated)	Wavelength (λmax) 418 ± 3 nm (Methanol)
Storage conditions	Freeze: Store at -5 to -30°C (F) Protect from moisture (D) Protect from light (L)
MDL#

* 4-Dimethylaminophenylazophenyl-4'-maleimide
* 4-Maleimido-4'-dimethylaminobenzene] [4-(4-dimethylaminophenylazo)phenylmaleimide
* 1H-Pyrrole-2,5-dione,1-(4-((4- (dimethylamino)phenyl)azo)phenyl)