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Catalog ID 7508 / 10 mg

5-FAM Alkyne *Single Isomer* *Click Chemistry*

AKA: 5-Carboxyfluorescein, Propargylamide
Formula Weight CAS#
C24H15NO6 413.38 N/A
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5-FAM alkyne is a green-fluorescent dye that is reactive with azides using copper catalyzed click chemistry. Click chemistry is favored for labeling and detection of target molecules because the highly selective labeling reaction is fast and efficient with few side products, requires no special solvents or temperatures, and yields a stable linkage between the target and the label. 5-FAM is a commonly used fluorescent label for proteins, peptides, and nucleic acids. 5-FAM alkyne is excited near 494 and has a pH dependent fluorescent emission near 519 nm. .

Product Size 10 mg
Form Orange Solid
Absorption (estimated) Wavelength (λmax) 494 ± 3 nm (Buffer pH 10)
Fluorescence (estimated) Wavelength (λmax) 519 ± 4 nm (*Buffer pH 10)
Storage conditions Freeze: Store at -5 to -30°C (F) Protect from moisture (D) Protect from light (L)
MDL#

* 5-Carboxyfluorescein, Propargylamide
* 2-(6-Hydroxy-3-oxo-3H-xanthen-9-yl)-5-(prop-2-yn-1-ylcarbamoyl)benzoic acid

Click Chemistry:   Alkyne
Fluorescent Probes and Related Products:   Fluorescein, Naphthofluorescein and seminaphthofluorescein

Safety Data Sheet
SDS-PDF
Certificates of Analysis
Lot 50503 27-Jun-2015
Lot 50438 06-May-2015

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