Product 6112
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Indo-1, AM is a cell-permeant, fluorescent ion indicator for intracellular calcium ion measurements in live cells. Indo-1’s fluorescence response to ion-binding permits a ratiometric calculation of calcium ion concentration that cancels artifacts of photobleaching, dye concentration gradients, cell thickness and dye leakage. When excited at 350 nm, Indo-1’s fluorescence emission wavelength shifts from near 475 nm to near 400 nm upon binding of calcium ions. Indo-1 has been used as a fluorescent calcium ion indicator in a wide variety of living cells and tissues and is well-suited for use in flow cytometry applications. We have formulated this convenient, ready-to-use 1 mM solution in DMSO and we also offer Indo-1 AM as a dried solid in 1 mg units (product 6080) and 20 x 50 ug special packaging (product 6085). .
Product Size | 1 mL |
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Form | Off white Solid |
Absorption (estimated) | Wavelength (λmax) 356 ± 3 nm (Methanol) |
Fluorescence (estimated) | Wavelength (λmax) 474 ± 4 nm (Methanol) |
Storage conditions | Freeze: Store at -5 to -30°C (F) Protect from moisture (D) Protect from light (L) |
MDL# |
* 4-(6-carboxy-2-indolyl)-4'-methyl-2,2'-(ethylenedioxy) dianiline-N,N,N',N'-tetraacetic acid acetoxymethyl ester
Cellular Sensors, Ion and pH Indicators (Fluorescent/Non-Fluorescent): | Fluorescent Calcium Indicators |
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Fluorescent Probes and Related Products: | Fluorescent Indicators (Ca, Cl, K, Mg, Na, Zn) |
Label or Dye: | Indo-1 and Mag-indo-1 |
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