Product 6322
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Catalog ID 6322 / 1 g
DiOC2(3)
AKA: 3,3'-Diethyloxacarbocyanine iodide
Formula | Weight | CAS# |
---|---|---|
C21H21IN2O2 | 460.31 | 905-96-4 |
For 25+ Units, Contact us.
DiOC2(3) is a lipophilic, green-fluorescent dye that is commonly used as a membrane potential probe in bacteria and yeasts. Cationic DiOC2(3) accumulates in hyperpolarized cell membranes and aggregates in the lipid bilayer. The formation dye aggregates within the cell membrane leads to a decreased fluorescence intensity and red-shifted fluorescence emission. The red-shifting fluorescence emission has been utilized in ratiometric techniques to estimate cell membrane potentials that are precise and accurate. The fluorescence of these environment-sensitive dyes is greatly enhanced when incorporated into membranes or bound to lipophilic biomolecules such as proteins. They are weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes. DiOC2(3) is excited near 482 nm and has an environmentally dependent fluorescence emission near 497 nm.
Product Size | 1 g |
---|---|
Form | Red solid |
Absorption (estimated) | Wavelength (λmax) 482 ± 3 nm (Methanol) |
Fluorescence (estimated) | Wavelength (λmax) 497 ± 4 nm (Methanol) |
Storage conditions | Store at Room Temperature (RT) Protect from moisture (D) Protect from light (L) |
MDL# | MFCD00011952 |
* 3,3'-Diethyloxacarbocyanine iodide
* 3-Ethyl-2-[3-(3-ethyl-2(3H)-benzoxazolylidene)-1-propenyl]benzoxazolium iodide
* 3-Ethyl-2-[3-(3-ethyl-2(3H)-benzoxazolylidene)-1-propenyl]benzoxazolium iodide
Fluorescent Probes and Related Products: | Carbocyanine/Styryl |
---|---|
Cell stains / Cellular Structure Probes: | Cell Membranes and Lipids |
Fluorescent/Non-Fluorescent Membrane and Membrane Potential Probes: | Membrane Potential Probes |
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