Product 6163
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Mag-fura-2, AM is a cell membrane-permeant, short-wavelength fluorescent ion indicator for intracellular magnesium ion measurements. Mag-fura-2’s spectral response to ion-binding permits a ratiometric calculation of ion concentration that cancels artifacts of photobleaching, dye concentration gradients, cell thickness and dye leakage. Live cells are passively loaded with Mag-Fura-2, AM by incubation with the cell-membrane permeant AM esters. Once internalized, cytosolic esterases cleave the AM esters to liberate the active tetra-carboxylate ligand. The excitation wavelength of Mag-fura-2 varies with Mg2+ concentration and shifts to shorter wavelengths with increasing Mg2+ concentration. The ratio of fluorescence intensity at 510 nm when excited at 340/380 nm is used for ratiometric calculation of ion concentration. Mag-fura-2 has also been used as a low affinity fluorescent indicator for intracellular Ca2+ measurements where high concentration Ca2+ transients would saturate higher affinity indicators. This is Setareh Biotech's high purity version of Mag-Fura-2, AM with HPLC purity greater than 98%. .
Product Size | 1 mg |
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Form | Lite Yellow Solid |
Absorption (estimated) | Wavelength (λmax) 366 ± 3 nm (Ethyl acetate) |
Fluorescence (estimated) | Wavelength (λmax) 472 ± 4 nm (Ethyl acetate) |
Storage conditions | Freeze: Store at -5 to -30°C (F) Protect from moisture (D) Protect from light (L) |
MDL# |
* Furaptra, AM ester
* 5-Oxazolecarboxylic acid, 2-[5-[2-[(acetyloxy)methoxy]-2-oxoethoxy]-6-[bis[2-[ (acetyloxy)methoxy]-2-oxoethyl]amino]-2-benzofuranyl] -(acetyloxy)methyl ester
* 5-Oxazolecarboxylic acid, 2-[5-[2-[(acetyloxy)methoxy]-2-oxoethoxy]-6-[bis[2-[ (acetyloxy)methoxy]-2-oxoethyl]amino]-2-benzofuranyl] -(acetyloxy)methyl ester
Cellular Sensors, Ion and pH Indicators (Fluorescent/Non-Fluorescent): | Fluorescent Calcium Indicators |
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Fluorescent Probes and Related Products: | Fluorescent Indicators (Ca, Cl, K, Mg, Na, Zn) |
Cellular Sensors, Ion and pH Indicators (Fluorescent/Non-Fluorescent): | Fluorescent Magnesium Indicators |
Label or Dye: | Fura-2 / Fura-4 / Fura-5 / Fura-FF / Mag-fura |
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