Product 6097
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Catalog ID 6097 / 500 ug
Fura-4F, pentapotassium salt *Cell impermeant*
Formula | Weight | CAS# |
---|---|---|
C28H19FK5N3O14 | 835.96 | 299172-08-0 |
For 25+ Units, Contact us.
Fura-4F, pentapotassium salt is a cell-impermeant, fluorescent ion indicator for intracellular calcium ion measurements. Fura-4F’s spectral response to ion-binding permits a ratiometric calculation of ion concentration that cancels artifacts of photobleaching, dye concentration gradients, cell thickness and dye leakage. The pentapotassium salt form of Fura-4F is water soluble and may be loaded into live cells by microinjection, patch pipette infusion, scrape loading, bead loading, or ATP-induced permeabilization. The excitation wavelength of Fura-4F varies with calcium ion concentration and shifts to shorter wavelengths with increasing calcium concentration. The ratio of fluorescence intensity at 510 nm when excited at 340/380 nm is used for ratiometric calculation of calcium ion concentration. Fura-4F is a lower affinity indicator for calcium and is useful for measurements of higher physiological concentrations of calcium ions where a higher affinity indicator would be fully saturated. .
Product Size | 500 ug |
---|---|
Form | Yellow-Solid |
Absorption (estimated) | Wavelength (λmax) 353 ± 3 nm (Methanol) |
Fluorescence (estimated) | Wavelength (λmax) 486 ± 4 nm (Methanol) |
Storage conditions | Freeze: Store at -5 to -30°C (F) Protect from moisture (D) Protect from light (L) |
MDL# |
* 5-Oxazolecarboxylic acid, 2-[6-[bis(carboxymethyl)amino]-5-[2-[2-[bis(carboxymethyl)amino]-4-fluorophenoxy]ethoxy]-2-benzofuranyl]-, pentapotassium salt]
Cellular Sensors, Ion and pH Indicators (Fluorescent/Non-Fluorescent): | Fluorescent Calcium Indicators |
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Fluorescent Probes and Related Products: | Fluorescent Indicators (Ca, Cl, K, Mg, Na, Zn) |
Label or Dye: | Fura-2 / Fura-4 / Fura-5 / Fura-FF / Mag-fura |
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