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Catalog ID 6064 / 25 mg

bBBR

AKA: Dibromobimane
Formula Weight CAS#
C10H10Br2N2O2 350.00 68654-25-1
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Dibromobimane is a low molecular weight, non-ionic, fluorescent probe for labeling thiols and crosslinking proteins. This cell-permeant probe is essentially non-fluorescent until both bromomethyl groups have reacted with thiols. The bromomethyl groups are relatively selective for reaction with thiols at physiological pH but may also react with carboxylates at pH above 7. Dibromobimane has been used to form an intramolecular-crosslink in myosin, hemoglobin, ATPase and other thiol-containing proteins. The conjugates of Dibromobimane are optimally excited near 394 nm and have a variable emission near 490 nm.

Product Size 25 mg
Form Yellow Solid
Absorption (estimated) Wavelength (λmax) 360 ± 3 nm (Methanol)
Fluorescence (estimated) Wavelength (λmax) 477 ± 4 nm (*Buffer pH 7)
Storage conditions Freeze: Store at -5 to -30°C (F) Protect from moisture (D) Protect from light (L)
MDL#

* Dibromobimane
* 1H,7H-Pyrazolo(1,2-?)pyrazole-1,7-dione, 3,5-bis(bromomethyl)-2,6-dimethyl-

Fluorescent Probes and Related Products:   Bimane
Reactive Probes and Related Products:   Thiol-Reactive (Haloacetamide/Halobenzyl and Maleimide)

Safety Data Sheet
SDS-PDF

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