Product 6114
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Catalog ID 6114 / 1 mg
Indo-1, pentapotassium salt *Cell Impermeant*
Formula | Weight | CAS# |
---|---|---|
C32H26K5N3O12 | 840.06 | 96314-96-4 |
For 25+ Units, Contact us.
Indo-1, pentapotassium salt is a cell-impermeant fluorescent ion indicator for intracellular calcium ion measurements in live cells. Indo-1’s fluorescence response to ion-binding permits a ratiometric calculation of calcium ion concentration that cancels artifacts of photobleaching, dye concentration gradients, cell thickness and dye leakage. When excited at 350 nm, Indo-1’s fluorescence emission wavelength shifts from near 475 nm to near 400 nm upon binding of calcium ions. Indo-1 has been used as a fluorescent calcium ion indicator in a wide variety of living cells and tissues and is well-suited for use in flow cytometry applications. The pentapotassium salt form of Indo-1 is water soluble and may be loaded into live cells by microinjection, patch pipette infusion, scrape loading, bead loading, or ATP-induced permeabilization. .
Product Size | 1 mg |
---|---|
Form | Off white Solid |
Absorption (estimated) | Wavelength (λmax) 348 ± 3 nm (Methanol) |
Fluorescence (estimated) | Wavelength (λmax) 473 ± 4 nm (Methanol) |
Storage conditions | Freeze: Store at -5 to -30°C (F) Protect from moisture (D) Protect from light (L) |
MDL# |
* 4-(6-carboxy-2-indolyl)-4'-methyl-2,2'-(ethylenedioxy)dianiline-N,N,N',N'-tetraacetic acid pentapostassium salt
Cellular Sensors, Ion and pH Indicators (Fluorescent/Non-Fluorescent): | Fluorescent Calcium Indicators |
---|---|
Fluorescent Probes and Related Products: | Fluorescent Indicators (Ca, Cl, K, Mg, Na, Zn) |
Label or Dye: | Indo-1 and Mag-indo-1 |
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