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Catalog ID 6018 / 25 mg

CHCC Acid

AKA: 6-Chloro-7-hydroxycoumarin-3-carboxylic acid
Formula Weight CAS#
C10H5ClO5 240.60 183736-74-5
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CHCC Acid is a non-activated, blue-fluorescent, amine-reactive dye for synthesis of blue-fluorescent biomolecules. The carboxylic acid may be reacted with primary amines on peptides, proteins, modified nucleotides, biopolymers and other amine-containing biomolecules using a carbodiimide like EDAC or DIPC. Carboxylic acids can also be used to synthesize ester-linked conjugates or acid-halide products. CHCC may also be used as building block for the synthesis of unique enzyme substrates and as a reference standard for enzymatic substrates synthesized from CHCC dye. CHCC is a short-wavelength fluorophore that is optimally excited near 390 nm with emission at 442 nm. The fluorescence of this dye is pH and environment dependent. .

Product Size 25 mg
Form Lite Yellow Solid
Absorption (estimated) Wavelength (λmax) 390 ± 3 nm (Buffer pH 10)
Fluorescence (estimated) Wavelength (λmax) 442 ± 4 nm (*Buffer pH 10)
Storage conditions Store at Room Temperature (RT) Protect from moisture (D) Protect from light (L)
MDL#

* 6-Chloro-7-hydroxycoumarin-3-carboxylic acid
* 2H-1-Benzopyran-3-carboxylic acid, 6-chloro-7-hydroxy-2-oxo-

Amine-Reactive Fluorophores, Biotins and Other Labels:   Carboxylic Acid (Non-Activated, free acid)
Fluorescent Probes and Related Products:   Coumarin (Umbelliferone)
Label or Dye:   Coumarin (Umbelliferone) and Methylcoumarin
Reactive Probes and Related Products:   Non-Activated, Amine-Reactive (Free Acid)

Safety Data Sheet
SDS-PDF
Certificates of Analysis
Lot 51871 22-Sep-2020
Lot 51564 01-Feb-2019

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