Product 6097

In Stock

Catalog ID 6097 / 500 ug

Fura-4F, pentapotassium salt Cell impermeant

Formula	Weight	CAS#
C₂₈H₁₉FK₅N₃O₁₄	835.96	299172-08-0

1-4 - ¤ 110/Unit 5-24 - ¤ 101/Unit

For 25+ Units, Contact us.

Fura-4F, pentapotassium salt is a cell-impermeant, fluorescent ion indicator for intracellular calcium ion measurements. Fura-4F’s spectral response to ion-binding permits a ratiometric calculation of ion concentration that cancels artifacts of photobleaching, dye concentration gradients, cell thickness and dye leakage. The pentapotassium salt form of Fura-4F is water soluble and may be loaded into live cells by microinjection, patch pipette infusion, scrape loading, bead loading, or ATP-induced permeabilization. The excitation wavelength of Fura-4F varies with calcium ion concentration and shifts to shorter wavelengths with increasing calcium concentration. The ratio of fluorescence intensity at 510 nm when excited at 340/380 nm is used for ratiometric calculation of calcium ion concentration. Fura-4F is a lower affinity indicator for calcium and is useful for measurements of higher physiological concentrations of calcium ions where a higher affinity indicator would be fully saturated. .

Product Size	500 ug
Form	Yellow-Solid
Absorption (estimated)	Wavelength (λmax) 353 ± 3 nm (Methanol)
Fluorescence (estimated)	Wavelength (λmax) 486 ± 4 nm (Methanol)
Storage conditions	Freeze: Store at -5 to -30°C (F) Protect from moisture (D) Protect from light (L)
MDL#

* 5-Oxazolecarboxylic acid, 2-[6-[bis(carboxymethyl)amino]-5-[2-[2-[bis(carboxymethyl)amino]-4-fluorophenoxy]ethoxy]-2-benzofuranyl]-, pentapotassium salt]

Cellular Sensors, Ion and pH Indicators (Fluorescent/Non-Fluorescent):	Fluorescent Calcium Indicators
Fluorescent Probes and Related Products:	Fluorescent Indicators (Ca, Cl, K, Mg, Na, Zn)
Label or Dye:	Fura-2 / Fura-4 / Fura-5 / Fura-FF / Mag-fura