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Catalog ID 7075 / 100 mg

    Other Unit Sizes:    7076 / 10 mL    7091 / 100 mg   

Hoechst 33258, Pentahydrate [bis-Benzimide]

AKA: Hoechst 33258 trihydrochloride, pentahydrate
Formula Weight CAS#
C25H37Cl3N6O 623.96 23491-45-4
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Hoechst 33258, Pentahydrate is a cell-permeant blue-fluorescent dye that exhibits a significant fluorescence enhancement upon binding to A-T regions in the minor grove of double stranded DNA. Hoechst dyes exhibit relatively large Stokes shifts (Excitation/Emission maxima near 350 nm/460 nm) and relatively low cytotoxicity making them suitable for multicolor labeling experiments in live cells. Hoechst 33258, Pentahydrate has been used in a variety of applications including nuclear counterstaining in immunofluorescent experiments, investigations of apoptosis pathways, and cell cycle studies.

Product Size 100 mg
Form Yellow-green solid
Absorption (estimated) Wavelength (λmax) 357 ± 3 nm (0.2M HCl/MeOH)
Fluorescence (estimated) Wavelength (λmax) 536 ± 4 nm (*0.2M HCl/MeOH)
Storage conditions Store at Room Temperature (RT) Protect from moisture (D) Protect from light (L)
MDL#

* Hoechst 33258 trihydrochloride, pentahydrate
* Bis-benzimide H 33258
* Phenol, 4-[5-(4-methyl-1-piperazinyl)[2,5'-bi-1H-benzimidazol] -2'-yl]-, trihydrochloride

Cell stains / Cellular Structure Probes:   DNA/RNA Cell Stains
Fluorescent Probes and Related Products:   Hoechst
Label or Dye:   Hoechst

Safety Data Sheet
SDS-PDF
Certificates of Analysis
Lot 50563 17-Aug-2015

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