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Catalog ID 6021 / 250 mg

3-Cyanoumbelliferone

AKA: 3-Cyano-7-hydroxycoumarin
Formula Weight CAS#
C10H5NO3 187.16 19088-73-4
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3-Cyanoumbelliferone is a blue-fluorescent dye that is commonly used as a reference standard in Cytochrome P450 enzymatic activity assays. The ethyl ether of 3-Cyanoumbelliferone, 3-Cyano-7-ethoxycoumarin (Product 6099), is a commonly used fluorescent substrate for Cytochrome P450 isoform CYP1A1. 3-Cyanoumbelliferone has a lower pKa than related coumarin dyes and is fluorescent at neutral pH, making this dye a preferred fluorophore for enzymatic assays performed at physiological pH. 3-Cyanoumbelliferone is excited at near 408 nm with emission near 450 nm.

Product Size 250 mg
Form Yellow-Orange solid
Absorption (estimated) Wavelength (λmax) 408 ± 3 nm (Buffer pH 10)
Fluorescence (estimated) Wavelength (λmax) 452 ± 4 nm (*Buffer pH 10)
Storage conditions Freeze: Store at -5 to -30°C (F) Protect from moisture (D) Protect from light (L)
MDL# MFCD00037480

* 3-Cyano-7-hydroxycoumarin

Fluorescent Probes and Related Products:   Coumarin (Umbelliferone)
Label or Dye:   Coumarin (Umbelliferone) and Methylcoumarin

Safety Data Sheet
SDS-PDF
Certificates of Analysis
Lot 51974 08-Mar-2021

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