Product 6134
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Catalog ID 6134 / 1 mg
Fura-2, pentapotassium salt *Cell Impermeant*
Formula | Weight | CAS# |
---|---|---|
C29H22K5N3O14 | 831.99 | 113694-64-7 |
For 25+ Units, Contact us.
Fura-2, pentapotassium salt is a cell-impermeant, fluorescent ion indicator for intracellular calcium ion measurements. Fura-2’s spectral response to ion-binding permits a ratiometric calculation of ion concentration that cancels artifacts of photobleaching, dye concentration gradients, cell thickness and dye leakage. The pentapotassium salt form of Fura-2 is water soluble and may be loaded into live cells by microinjection, patch pipette infusion, scrape loading, bead loading, or ATP-induced permeabilization. The excitation wavelength of Fura-2 varies with calcium ion concentration and shifts to shorter wavelengths with increasing calcium concentration. The ratio of fluorescence intensity at 510 nm when excited at 340/380 nm is used for ratiometric calculation of calcium ion concentration. This is the pentapotassium salt form of Fura-2 for use in applications that may be perturbed by sodium ions. .
Product Size | 1 mg |
---|---|
Form | Yellow Solid |
Absorption (estimated) | Wavelength (λmax) 354 ± 3 nm (Methanol) |
Fluorescence (estimated) | Wavelength (λmax) 457 ± 4 nm (Methanol) |
Storage conditions | Freeze: Store at -5 to -30°C (F) Protect from moisture (D) Protect from light (L) |
MDL# |
* 5-Oxazolecarboxylic acid, 2-(6-(bis(carboxymethyl)amino)-5-(2-(2-(bis(carboxymethyl)amino)-5-methylphenoxy)ethoxy)-2-benzofuranyl)-, pentapotassium salt
Cellular Sensors, Ion and pH Indicators (Fluorescent/Non-Fluorescent): | Fluorescent Calcium Indicators |
---|---|
Fluorescent Probes and Related Products: | Fluorescent Indicators (Ca, Cl, K, Mg, Na, Zn) |
Label or Dye: | Fura-2 / Fura-4 / Fura-5 / Fura-FF / Mag-fura |
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