Product 8058
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biotinylation is the process of covalently attaching biotin to a protein, nucleic acid or other molecule. Biotinylation is rapid, specific and is unlikely to perturb the natural function of the molecule due to the small size of biotin (MW = 244.31). Biotin binds to streptavidin and avidin with an extremely high affinity, fast on-rate, and high specificity, and these interactions are exploited in many areas of biotechnology to isolate biotinylated molecules of interest. N-hydroxysuccinimide (NHS) esters have poor solubility in aqueous solutions. For reactions in aqueous solution, they must first be dissolved in an organic solvent, then diluted into the aqueous reaction mixture. The most commonly used organic solvents for this purpose are dimethyl sulfoxide (DMSO) and dimethyl formamide (DMF), which are compatible with most proteins at low concentrations. Because of the hydrophobicity of NHS-esters, NHS biotinylation reagents can also diffuse through the cell membrane, meaning that they will biotinylate both internal and external components of a cell.
Product Size | 100 mg |
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Form | White Solid |
Storage conditions | Freeze: Store at -5 to -30°C (F) Protect from moisture (D) Protect from light (L) |
MDL# | MFCD00078531 |
* D-Biotin, succinimidyl ester [Succinimidyl D-biotin]
* Link-EZ NHS-Biotin
* EZ-Link NHS-Biotin *TM of ThermoFisher Scientific / Pierce Biotech* [D-Biotin SE] [2,5-pyrrolidinedione, 1-((5-hexahydro-2-oxo-1H-thieno-(3,4-d)-imidazol-4-yl)-1-oxopentyl)oxy)-(3aS-(3a-,4,6a-))-]
* Link-EZ NHS-Biotin
* EZ-Link NHS-Biotin *TM of ThermoFisher Scientific / Pierce Biotech* [D-Biotin SE] [2,5-pyrrolidinedione, 1-((5-hexahydro-2-oxo-1H-thieno-(3,4-d)-imidazol-4-yl)-1-oxopentyl)oxy)-(3aS-(3a-,4,6a-))-]
Reactive Probes and Related Products: | Activated, Amine-Reactive [Isothiocyanate, Succinimydyl Ester (SE), Sulfonyl Halide, TFP Ester, NBD-Cl, NBD-F, etc.] |
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Amine-Reactive Fluorophores, Biotins and Other Labels: | Active Ester - Succinimidyl Ester (SE or NHS-ester) |
Biotin/Desthiobiotin and Related Products: | Amine-Reactive Probes [Succinimydyl Ester (SE), NHS-ester or Free Acid] |
Label or Dye: | Biotin, Biotin Analogs, Biotin-X and Biotin-XX |
Product Line: | Link-EZ (Known as EZ-Link, TM of ThermoFisher Scientific / Pierce Biotech) |
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