Product 6099

Lead Time 2 Days

Catalog ID 6099 / 10 mg

3-Cyano-7-ethoxycoumarin Cytochrome P450 Substrate

AKA: 7-Ethoxycoumarin-3-carbonitrile

Formula	Weight	CAS#
C₁₂H₉NO₃	215.21	117620-77-6

1-4 - ¤ 94/Unit 5-24 - ¤ 89/Unit

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3-Cyano-7-ethoxycoumarin is a fluorogenic substrate useful in microsomal dealkylase studies and for the continuous determination of cytochrome P450 mixed-function monooxygenases. 3-Cyano-7-ethoxycoumarin is essentially non-fluorescent until enzymatic cleavage of the alkyl ether liberates the fluorescent product. The pKa of 3-Cyano-7-hydroxycoumarin (product 6021) is lower than related 7-hydroxycoumarin substrates and makes this probe useful for measurements of enzymatic activity at pH 7. The enzymatically dealkylated fluorescent product is excited near 408 nm with fluorescence emission near 450 nm. .

Product Size	10 mg
Form	Yellow Solid
Storage conditions	Refrigerate: Store at 4-8°C (Ref) Protect from moisture (D) Protect from light (L)
MDL#	MFCD00467359

* 7-Ethoxycoumarin-3-carbonitrile
* 2H-1-Benzopyran-3-carbonitrile, 7-ethoxy-2-

Fluorescent Probes and Related Products:	Coumarin (Umbelliferone)
Label or Dye:	Coumarin (Umbelliferone) and Methylcoumarin
Enzyme Detection, Substrates and Activity Assays :	Cytochrome P450 Substrates