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Catalog ID 9199 / 5 mg

DBF *Cytochrome P450 Substrate*

AKA: Dibenzylfluorescein
Formula Weight CAS#
C34H24O5 512.55 97744-44-0
For 25+ Units, Contact us.

DBF is dealkylated by cytochromes P450 (especially CYP2C8, CYP2C9, CYP2C19, and CYP3A) to form a fluorescein ester. Dibenzylfluorescein was reported to be suitable for in vitro assessment of the initial screening of CYP3A4- mediated drug interactions.  The fluorescent fluorescein product can be quantitated after the addition of base, which hydrolyzes the ester and increases the pH for optimal fluorescein fluorescence detection, which hydrolyzes the ester and increases the pH for optimal fluorescein fluorescence detection.

Product Size 5 mg
Form Orange Solid
Absorption (estimated) Wavelength (λmax) 458 ± 3 nm (Methanol)
Storage conditions Freeze: Store at -5 to -30°C (F) Protect from moisture (D) Protect from light (L)
MDL# MFCD13184323

* Dibenzylfluorescein
* Benzyl 2-(6-(benzyloxy)-3-oxo-3H-xanthen-9-yl)benzoate
* NSC 645658

Enzyme Detection, Substrates and Activity Assays :   Cytochrome P450 Substrates
Fluorescent Probes and Related Products:   Fluorescein, Naphthofluorescein and seminaphthofluorescein

Safety Data Sheet
SDS-PDF
Certificates of Analysis
Lot 52377 25-May-2023
Lot 50873 13-Jun-2016

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