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Product 6181

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Catalog ID 6181 / 1 mg

    Other Unit Size:     7913 / 20 X 50 ug   

Calcein blue, AM *Cell permeant*

Formula Weight CAS#
C21H23NO11 465.41 168482-84-6

Calcein Blue, AM is a cell-permeant, blue-fluorescent, cell viability probe and polar tracer dye for short-term staining of live cells. Live cells are passively loaded with weakly fluorescent Calcein Blue, AM by incubation with the cell membrane-permeant AM esters. Once internalized, cytosolic esterases cleave the AM esters to liberate the dicarboxylate, polar tracer dye. Calcein Blue’s fluorescence is enhanced after enzymatic hydrolysis and the excitation and emission wavelengths shift to slightly longer wavelengths, 360 / 449 nm respectively. Caclein Blue AM is intrinsically fluorescent and Calcein Blue stained cells may require an extra wash step to reduce background fluorescence. .

Product Size 1 mg
Form beige solid
Absorption (estimated) Wavelength (λmax) 322 ± 3 nm (Methanol)
Fluorescence (estimated) Wavelength (λmax) 440 ± 4 nm (Methanol)
Storage conditions Freeze: Store at -5 to -30°C (F) Protect from moisture (D) Protect from light (L)
MDL#

Cell Analysis Reagents and Kits:   Cell Proliferation
Cell Analysis Reagents and Kits:   Cell Viability
Cell Analysis Reagents and Kits:   Cell, Tissue and In Vivo Labeling Kits and Reagents
Enzyme Detection, Substrates and Activity Assays :   Esterase Substrates

Safety Data Sheet
SDS PDF
Certificates of Analysis
Lot 50961 15-Sep-2016

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