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Catalog ID 7091 / 100 mg

    Other Unit Sizes:    7075 / 100 mg    7076 / 10 mL   

Hoechst 33258, Pentahydrate [bis-Benzimide] *X-Pure*

AKA: Hoechst 33258 trihydrochloride, pentahydrate
Formula Weight CAS#
C25H37Cl3N6O 623.96 23491-45-4
For 25+ Units, Contact us.

We guarantee that *X-Pure* Grade Hoechst 33258 is greater than or equal to 98% pure by HPLC. The Hoechst 33258, 33342 and 34580 are cell permeant minor groove –binding DNA stains that are excited by UV light and fluoresce bright blue upon binding to DNA. Hoechst dyes exhibit relatively large Stock shifts (Excitation/Emission maxima ~350/460) making them suitable for multicolor labeling experiments. They are used in many cellular applications, including Cell-Cycle, apoptosis studies and they are common nuclear counterstains. We also offer other Hoechst dyes, special packaging and Extra Pure.

Product Size 100 mg
Form Yellow-green solid
Absorption (estimated) Wavelength (λmax) 357 ± 3 nm (0.2M HCl/MeOH)
Fluorescence (estimated) Wavelength (λmax) 536 ± 4 nm (*0.2M HCl/MeOH)
Storage conditions Store at Room Temperature (RT) Protect from moisture (D) Protect from light (L)
MDL#

* Hoechst 33258 trihydrochloride, pentahydrate
* Bis-benzimide H 33258
* Phenol, 4-[5-(4-methyl-1-piperazinyl)[2,5'-bi-1H-benzimidazol] -2'-yl]-, trihydrochloride

Cell stains / Cellular Structure Probes:   DNA/RNA Cell Stains
Fluorescent Probes and Related Products:   Hoechst
Label or Dye:   Hoechst

Safety Data Sheet
SDS-PDF
Certificates of Analysis
Lot 52444 26-Oct-2023

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